The invention discloses a hybridization method of wild paddy rice. A mature wild paddy rice hybridization technology is developed in the aspects of reasonably processing the different flowering phases of different materials, cautiously performing the castration operation, using different hybridization tools, controlling appropriate environmental conditions, identifying the hybridization and the like. The experiment proves that the success rate of the hybridization technology is high, more hybridized seeds can be obtained, the hybridization technology can be widely suitable for the hybridization requirements of different types of wild paddy rice and between the wild paddy rice and the cultivated paddy rice, the mining of genetic resources of the wild paddy rice can be facilitated, and contribution can be made to the diversified scientific research of the paddy rice.