The invention, which belongs to the field of the LED driving technology, discloses a method and system for pulse width segmentation of an LED drive. A clock period corresponding to one display period of an LED drive pulse corresponding to grayscale data is segmented into a first preset number of display sub periods and each display sub period is divided into a second preset number of display unit periods, wherein each display unit period includes a third preset number of clock pulse numbers; on the basis of the clock pulse number and the grayscale data, the number of the display unit period included by one display period of the LED drive pulse is obtained and the LED drive pulse is distributed in the display sub periods based on the number of the display unit periods. Therefore, while the LED refresh rate is ensured, the authenticity of the grayscale data corresponding to the LED drive pulse is not reduced, so that a problem that the high LED refresh rate and good display authenticity can not be realized simultaneously by the existing LED pulse width segmentation method is solved.