The present invention provides a scanning signal generation method and device, and a display device, and applied to the display technology field. The method comprises: providing conduction signals; providing turn-off signals, wherein the turn-off signals are controlled to jump to a preset voltage according to the type of a switching transistor in the black insertion phase of a pixel driving circuit; and generating scanning signals according to the conduction signals and the turn-off signals. In the black insertion phase, the turn-off signals are jumped to the preset voltage in a preset mode according to the type of the switching transistor so that even if the threshold voltage of the switching transistor generates negative drifting, the turn-off signals after jumping can turn off the switching transistor after the threshold voltage generates negative drifting to prevent charges in a capacitor from electric leakage through the switching transistor, allow the display brightness of electroluminescent cells to be consistent and avoid generation of cross color and splash screen phenomenons.