The invention discloses a reusable bottom clamping bolt. According to the technical scheme, the reusable bottom clamping bolt is characterized by comprising a fixing cylinder with one open end, a fastening block is connected in the fixing cylinder in a threaded mode, and the fastening block extends into the fixing cylinder and is provided with a circular-truncated-cone-shaped supporting block; a plurality of limiting columns are arranged on the side wall of the fixing cylinder in a penetrating mode, one end of each limiting column is provided with a pushing plate making contact with the side wall of the supporting block, and the limiting columns are sleeved with springs respectively. One end of each spring abuts against the fixing cylinder, and the other end of each spring abuts against apushing block; a threaded rod is arranged in the fastening block in a penetrating mode, and the threaded rod is sleeved with a positioning plate and a fastening nut; the outer wall of the fixing cylinder and the parts, protruding out of the outer wall of the fixing cylinder, of the limiting columns are coated with a hot melt adhesive. The bottom clamping bolt has the advantage of being reusable.