The invention provides a tracheal intubation method for big and small mice. The method includes the following steps of preparing animals and instruments; putting a simple laryngoscope into an oral cavity of a big mouse or a small mouse to expose the glottis of the big mouse or the small mouse; inserting a tracheal catheter into the trachea of the big mouse or the small mouse under orthophoria; connecting the tail end of the tracheal catheter with a breathing machine for small animals and an anesthesia machine for the small animals, and continuing conducting inhalation anesthesia and mechanicalventilation. The tracheal intubation method for the big and small mice is wide in application range, high in success rate, applicable to molding of the big and small mice different in size, medicineapplication for tracheas, lung function detection and other occasions, and capable of avoiding the situation the the success rate of blind intubation is low, and damage is caused to the bodies of animals; the applied instruments are adaptive to the characters that the glottises of the big and small mice are narrow and the tracheas of the big and small mice are located at the deep portions of the oral cavities, pain caused by organic damage during intubation is reduced, and the stress reaction of the animals to the intubation is small; the operation is simple, and one person can complete all operation.