The invention discloses a quick reader for identification of a serum antibody and a using method of the quick reader. The quick reader comprises n working elements and a box body, wherein each of theworking elements comprises a knob and a screening body; two ends of each of the screening bodies are connected with side walls of the box body by shafts; one end surface of each of the knobs is connected with one shaft of each of the screening bodies, and the screening bodies can be driven to rotate; each of the screening bodies is formed by connecting m screening units; a screening hole penetrating an axis of a pair of opposite side surfaces of each of the screening units is formed in each of the opposite side surfaces; a plurality of observation holes are formed in each of two side surfacesof the box body in the arrangement direction of the working elements; and the observation holes correspond to the positions of the m screening units. The quick reader is simple in structure and convenient to operate, an identification result is obtained through the observation holes only by inputting a reaction pattern, a sample antibody can be determined quickly and accurately, and a subjective false risk in a contrast analysis process is excluded.