The invention discloses a biological fermentation process for using chicken dung in feed production; chicken dung protein feed with high protein, high amino acid content, low cellulose content and high feed return rate is developed by integrating a modern mixed bacteria microbial fermentation technology; the process is simple and efficient in fermentation technology, good in deodorization and sterilization effects, low in production cost and easy in realization of industrial large-scale production. The application adopts two steps of fermentation processes, i.e., mixed bacteria aerobic fermentation and mixed bacteria anaerobic fermentation, wherein an aerobic fermentation microflora comprises mold, yeast, bacillus subtilis, thermophilic heterotrophic ammonia-oxidizing bacteria, and an anaerobic fermentation microflora comprises one or a mixture of more of streptococcus thermophilus, enterococcus faecium and lactobacillus acidophilus. After the process is adopted, complex metabolism, which is difficult to accomplish by means of a single strain, is completed by adopting mixed bacteria fermentation and combining different metabolic abilities, and the chicken dung is effectively transformed by means of synergistic fermentation of microorganisms; the hygienic indexes meet the standard requirements of China, and the product nutritional value indexes are obviously improved.