The invention provides a urine occult blood test paper and a production method thereof, and belongs to the field of urine detection. The test paper comprises a substrate and a filter paper fixed on the substrate, and the filter paper is obtained through performing immersion in a solution A and then performing immersion n a solution B; the solution A comprises a buffering solution, purified water and a surfactant A; the solution B comprises ethanol, a surfactant B, a toner, peroxide and a sensitizer; and the structure of the peroxide is represented by formula I. The invention also provides a production method of the urine occult blood test paper. The lowest detection concentration of the occult blood quick test paper produced through the method is 4 Cells/[mu]L, the detection range can reach 4-200 Cells/[mu]L, and the test paper also has the advantages of stable performances, fast test speed and accurate determination result.