The invention discloses an in-vitro training and testing system for percutaneous coronary interventional surgery. The in-vitro training and testing system comprises an operation platform, a constant-temperature box, a display, a sealed constant-temperature blood collecting box and a heart simulating device, wherein the constant-temperature box simulates the in-vivo environment of a human body, andthe display is connected with the constant-temperature box; the heart simulating device comprises a sealed rigid container, a piston communicated with the rigid container and a heart model arranged in the rigid container; the heart model is connected with an aorta model and a right pulmonary vein simulating pipeline communicated with the blood collecting box; left and right coronary artery modelsare connected to one end of the aorta model, and the far end of each coronary artery model is communicated with the blood collecting box through a silica-gel soft tube; the other end of the aorta model is communicated with one end of an artery simulating tube, the other end of the artery simulating tube is communicated with one end of a femoral artery blood vessel simulating section, and the other end of the femoral artery blood vessel simulating section is communicated with the blood collecting box. The in-vitro training and testing system has the advantages that the interventional surgery scene is restored, the surgery learning time of doctors is shortened, and the operation level of the doctors can be improved.