The invention provides a huge lipidosome preparing and sorting device which comprises a preparing device and a sorting device communicating to each other, wherein the preparing device comprises an upper piezoelectric ceramic wafer and a lower piezoelectric ceramic wafer which are arranged opposite to each other; an upper electrode is arranged in the middle of the upper piezoelectric ceramic wafer;a lower electrode opposite to the lower electrode is arranged on the lower piezoelectric ceramic wafer; an insulating substrate is arranged on one side, close to the upper electrode, of the lower electrode; a microporous array for preparing a huge lipidosome is arranged on the insulating substrate; the upper electrode is located above the microporous array; and the sorting device comprises an upper base and a lower base, and a microcolumn array for sorting the huge lipidsome is arranged between the upper base and the lower base. By means of the microporous array and the microcolumn array combined with the electrodes and the piezoelectric ceramic wafers, the prepared huge lipidosome is wide in grain size distribution and great in difference and good in preparing repeatability. The preparation efficiency and the sorting effecting are improved obviously, the lipidosome is unlikely to be broken, and the sorted huge lipidosome is good in grain size uniformity.