An enzyme-linked immunosorbent assay kit for detecting a sheep pox virus antibody and applications thereof are disclosed. The kit includes a coated antigen that is recombinant sheep pox virus fusion protein. The recombinant sheep pox virus fusion protein is a) protein the amino acid sequence of which is number 8-629 amino acid residues of SEQ ID No.2, or b) protein the amino acid sequence of whichis the SEQ ID No.2, or c) protein obtained by subjecting an amino acid sequence shown as the SEQ ID No.2 to substitution and/or deletion and/or addition of one or a plurality of amino acid residues and having activity same as activity of the protein described in a). The method of detecting the sheep pox virus antibody by utilizing the kit constructed by adopting the recombinant sheep pox virus fusion protein as the coated antigen can be used for diagnosing sheep pox, and can evaluate vaccine immunization effects, and can rapidly and accurately detect the sheep pox.