The invention relates to the field of organic synthesis, and discloses a bis-Schiff base compound as well as a preparation method and application thereof. The compound has a structure represented by aformula (1) in the description, wherein R1 is at least one group selected from the group consisting of methyl, ethyl, n-propyl, isopropyl, primary butyl, sec-butyl, tert-butyl, methoxyl, ethoxyl, n-propoxy, isopropoxy, primary butoxy, sec-butoxy and tert-butoxy; R2 is at least one group selected from the group consisting of methyl, ethyl, n-propyl, isopropyl and a halogen; and X is a halogen. Thebis-Schiff base compound provided by the invention has a novel structure, and the function of inhibiting the growth of bacteria, and the bis-Schiff base compound provided by the invention has strongability of complexing with metal ions.