The invention provides a high-quality preservation method for a cordyceps militaris strain. The method is characterized in that the cordyceps militaris strain is preserved in a form of bacterial suspension or sclertium under the condition that the temperature is 4 to 25 DEG C, and the preservation period can reach 12 months or above. According to the preservation method disclosed by the invention,quality and stability of the cordyceps militaris strain are improved by optimizing preservation conditions of a preservation form, protecting agent types, temperature range, time period and the likeof the cordyceps militaris strain, and technical guarantee is provided for strain preservation, artificial cultivation and the like.