The invention discloses application of KB-R7943 or Bepridil in preparing a medicine for treating gliomas. The invention discloses a novel strategy in treating gliomas, that is, gliomas are treated byusing a sodium-calcium permutoid inhibitor KB-R7943 or Bepridil, and an old medicine has new application. KB-R7943 and Bepridil have tumor cell killing mechanisms which are completely different from those of conventional medicines, and have no drug resistance as well; in addition, both KB-R7943 and Bepridil are capable of improving sensitivity of gliomas to other common chemotherapeutic drugs; dueto adoption of the two novel medicines, prognosis of patients suffering from gliomas can be improved, the survival rate of the patients can be increased, the social burden can be alleviated, and goodprospects can be achieved for treatment on gliomas.