The invention relates to an arterial puncture guide device and a use method thereof, and belongs to the technical field of medical equipment. The device comprises a bottom frame, a vertical column anda bearing platform, the vertical column and the bearing platform are fixed on the bottom frame, a vibration detector is fixed at the upper end of the vertical column by the aid of a cantilever and positioned above the bearing platform, a first strain gage is pasted on the surface of the vibration detector, an information output end of the first strain gage is connected with a strain meter, the vibration detector is horizontally overhung, the tail end of the vibration detector is a downward projection detection contact, the bearing platform bears the position to be punctured of a patient, thedetection contact contacts with the surface of the position to be punctured by the aid of a position adjusting system, the position adjusting system is arranged between the bearing platform and the bottom frame and/or the vibration detector and a cantilever, and the position adjusting system comprises an X-Y-Z linear displacement mechanism and an X-Y-Z overturning mechanism. According to the device, artery positions can be rapidly and accurately found, a lot of time and labor are saved, and dangerous conditions such as later needle deviation and purple stasis can be avoided as the device has high accuracy.