The invention relates to application of pterostilbene in preparation of an NDM-1 enzyme inhibitor, and discloses new medical application of the pterostilbene. The pterostilbene can inhibit the activity of an NDM-1 enzyme, and recover the bactericidal activity of meropenem to NDM-1 escherichia coli; in an in-vivo experiment, the pterostilbene and the meropenem are combined to have a good treatmenteffect on infection of bacteria for expressing NDM-1, especially infection such as peritonitis caused by NDM-1 positive escherichia coli, and the pterostilbene has wide medical application.