The invention discloses a method for inducing and producing corn female parent haploid. The method for inducing and producing the corn female parent haploid, provided by the invention, comprises the following steps: silencing or inhibiting expression of a ZmPLA gene in a target plant genome or knocking out a PLA (Polylactic Acid) gene to obtain a transgenic plant or an offspring thereof; obtainingthe corn female parent haploid in a selfing process or a process of taking the transgenic plant or the offspring thereof as a male parent to be hybridized with other materials. An experiment disclosed by the invention proves that mutation of ZmPLA can cause to production of the corn female parent haploid and an important foundation is laid for disclosing genetic and biological mechanisms for producing the corn female parent haploid. Meanwhile, a mutant single plant obtained by utilizing the experiment or the method has a haploid induction capability of a corn female parent and has great significance of selectively breeding a novel induction system, further improving the induction rate and improving the breeding efficiency of the corn haploid.