The invention discloses application of human GRK5 genes, namely application of human GRK5 genes serving as cancer cell specified action targets in preparation of drugs for treating non-small cell lungcancer. The cancer cell specified action targets are RNA interference effect targets. Compared with normal pulmonary bronchial epithelial cells, the GRK5 genes have high expressions in the non-smallcell lung cancer cells; RNA interfering lentivirus designed aiming at the GRK5 genes can inhibit GRK5 expression and is capable of obviously inhibiting proliferation of the non-small cell lung cancercells, blocking tumor cell division at a G2/M phase, promoting cells apoptosis, inhibiting cell migration and inhibiting formation of xenograft tumor in nude mice, and the aim of treating the non-small cell lung cancer can be further achieved. The human GRK5 genes can serve as treatment targets of the non-small cell lung cancer, and provide wide prospects for developing medicines in treatment of the non-small cell lung cancer based on the GRK5 genes in future.