The invention provides a method used for comprehensive determination of carbonate rock stratum system hydrocarbon source rock hydrocarbon supply threshold. The method comprises following steps: basedon the change relationship of hydrogen to carbon atom ratio H/C and R, fitting of a H/C value evolution curve is carried out, the R corresponding to H/C=0.01 is taken as a hydrocarbon supply threshold R1; based on the change relationship of oxygen to carbon atom ratio O/C and R, fitting of an O/C value evolution curve is carried out, the R corresponding to O/C=0.02 is taken as a hydrocarbon supply threshold R2; based on the change relationship of hydrocarbon generation potential index (S1+S2)/TOC and R, fitting of a hydrocarbon expulsion is carried out, the R corresponding to the last 1% hydrocarbon generation amount is taken as a hydrocarbon supply threshold R3; the hydrocarbon supply threshold range is determined based on the largest value and the smallest value selected from R1, R2, and R3; and based on the change relationship of different region R and depth H, corresponding hydrocarbon supply threshold range is determined, and the hydrocarbonsupply threshold depth range of different regions is obtained.