The present invention discloses a test method of researching an influence of a pressing force of a transformer on winding vibration. The method comprises the following steps that: a vibration signal measurement system detects vibration signals at the surface of a transformer winding through a vibration acceleration sensor; determining whether a current frequency on the transformer winding is larger than or equal to a preset current frequency value or not, and when the current frequency on the transformer winding is smaller than the preset current frequency value, the current frequency on the transformer winding adds BHz; when axial pressing force of the transformer winding is larger than a preset pressing force, the axial pressing force of the transformer winding reduces AN, the current frequency of the transformer winding and an effective value are respectively regulated to an initial current frequency and an effective value; a frequency spectrum analysis method is employed to performprocessing of obtained vibration signals and extract fundamental frequency vibration amplitudes in a frequency spectrum to obtain frequency response features of fundamental frequency vibration of thetransformer winding under different axial pressing forces. The test method of the researching influence of the pressing force of the transformer on winding vibration can obtain frequency response features of fundamental frequency vibration of the transformer winding under different axial pressing forces.