The invention discloses six types of structures of 1, 5-aza-spiro [2, 4] heptanes alkaloid and application thereof. The 1, 5-aza-spiro [2, 4] heptanes alkaloid is of novel structures. Vinyl azide is used as a raw material for the 1, 5-aza-spiro [2, 4] heptanes alkaloid, and the 1, 5-aza-spiro [2, 4] heptanes alkaloid can be prepared by means of intramolecular Alder ene reaction. The structures andthe application have the advantages that human cervical cancer cell proliferation inhibition effects of partial compounds of the 1, 5-aza-spiro [2, 4] heptanes alkaloid are measured by the aid of MTT(methyl thiazolyl tetrazolium) processes, and the potential antitumor activity of the 1, 5-aza-spiro [2, 4] heptanes alkaloid is shown by results.