The invention discloses application of hydroquinoline compounds in enhancing the sensitivity of bacteria to polymyxin. Experiments on the minimum inhibitory concentration of multiple MCR-1 positive drug resistant bacteria find that the hydroquinoline compounds can re-sensitize MCR-1 positive drug resistant bacteria to polymyxin, which provides a strong theoretical basis and practical basis for thefurther development of new strategies for treating MCR-1 positive drug resistant bacteria and for promoting the clinical transformation of therapeutic drugs. The application has important research and development value and development significance.