The embodiment of the invention provides a fixture and an electronic device screw disassembling and assembling method. The fixture comprises a base body, a first cover plate and a second cover plate;the base body is provided with a containing groove used for containing electronic device; the first cover plate is connected with the base body, used for positioning the electronic device into the containing groove, and provided with a containing hole penetrating through the first cover plate in the thickness direction of the first cover plate, a plurality of first guide holes for disassembling the electronic device are formed in the first cover plate and correspond to screws of the electronic device; and the second cover plate is arranged in the containing hole, connected with the first coverplate, and used for positioning the electronic device into the containing groove. According to the embodiment of the invention, the electronic device screw disassembling and assembling efficiency canbe improved.