The invention discloses an application of Application of protective medicine for ischemia reperfusion injury prepared by mitochondrial complex I reversibility inhibitor and active oxygen scavenger. According to the invention, the mitochondrial complex I reversibility inhibitor can play a protective role similar to ischemic preconditioning, and can be used as an ischemic preconditioning medicament;the mitochondrial complex I reversibility inhibitor is combined with active oxygen scavenger, the mitochondrial complex I is administered reversibility inhibitor before ischemia, and is administeredactive oxygen scavenger before reperfusion, and the therapeutic effect of ischemia reperfusion can be obviously improved. According to the invention, the double-layer tablet is taken before ischemia,the outer quick release layer containing the mitochondrial complex I reversibility inhibitor quickly releases and play the role of ischemic preconditioning, the inner slow release layer containing reactive oxygen scavenger slowly releases and reaches the maximum plasma concentration before reperfusion, so as to improve the curative effect of ischemia reperfusion, and the medicine is effective andconvenient.