The invention discloses an MCR-1 inhibitor and application thereof in preparing medicine for inhibiting MCR-1 positive drug-resistant bacteria. An MCR-1 inhibitor screening system is used, it is proved that the MCR-1 inhibitor (a methylurea compound modified by halogenated benzyl and pyrazole methyl) can inhibit the proliferation activity of the MCR-1 positive drug-resistant bacteria, and the resensitization of the MCR-1 positive drug-resistant bacteria to polymyxin is inhibited. The MCR-1 inhibitor provides powerful theoretical bases and practical bases for further promoting the research anddevelopment of new strategies for the treatment development of the MCR-1 positive drug-resistant bacteria and promoting the clinical transformation of the treatment medicine and has high development value and important development significance.