The invention discloses chicken circRNA (Circular RNA). The nucleotide sequence of the chicken circRNA is shown in SEQ ID NO: 2. The invention also provides detection primers of the chicken circRNA, and the nucleotide sequences of the detection primers are shown in SEQ ID NO: 3-4. Existence of circular transcript CircGHR of GHR is verified, full length of the molecular sequence of the circRNA is obtained by cloning, the CircGHR ring structure is determined, and the molecule has resistance to nucleic acid exonuclease RNase R. Quantitative results show that with growth and development of the body, CircGHR presents different trends, gradually decreases in pectoral muscle and leg muscle tissue and is highly expressed in the myoblast nucleus, indicating that CircGHR has an inhibitory effect onthe muscle development process of Huaixiang chicken after hatching. Meanwhile, the application of chicken circRNA CGHR in detection or evaluation of the chicken muscle rate also has great applicationvalue.