The invention belongs to the technical field of a wood board adhesive and in particular relates to a preparation method of semi-solid bamboo liquid-protein hot pressboard glue. The preparation methodcomprises the following steps: tanking phenol and concentrated sulfuric acid to react to obtain red water-soluble sulfonated phenol; crushing bamboos until the size is 80 meshes and adding into the sulfonated phenol; supplementing a catalyst and water to obtain brownish red solid-containing viscous liquid; adding powdery protein to obtain viscous brownish red solid-containing viscous liquid; adding soda ash and regulating the pH (Potential of Hydrogen); adding a regulator to obtain brownish red viscous solid-containing glue. According to the preparation method, bamboo fibers and protein polypeptide form a Maillard reaction crosslinking agent in a hot pressing process to obtain an adhesion force adhesive; the functions of no formaldehyde, no volatile solvents and relatively good water resistance are realized.