The invention discloses a door and window sealing structure. The door and window sealing structure comprises door and window glass, an aluminum frame, a plastic frame, a first rotating shaft and firstwheels; first grooves are formed in the two ends of the plastic frame, the two ends of the top of the aluminum frame are connected with aluminum strips correspondingly, second grooves are formed in the upper portions of the inner sides of the aluminum strips, and the first wheels are located in the second grooves; second adhesive tapes are connected to the two ends in the aluminum frame correspondingly, the inner sides of the second adhesive tapes make mutual contact with the two ends of the plastic frame, the second adhesive tapes are located on the lower portions of the aluminum strips, gaps are formed between the second adhesive tapes and the aluminum strips, and the interiors of the gaps are movable connected with spacing plates through a plurality of springs; the outer side of each spacing plate is connected with a row of first balls, each spacing plate is further provided with a row of through holes, and the through holes make mutual contact with the first balls; the outer sidesof the first balls are located in the first grooves; and the interior of the aluminum frame is further connected with a row of second rotating shafts, and the second rotating shafts are in shaft connection with second wheels. According to the door and window sealing structure, the defects of the prior art can be improved, and a sealing part of the door and window sealing structure has better reliability.