The invention provides an over-current protection self-locking circuit and an air conditioner. By arranging a voltage changing unit, a first comparator can be enabled to maintain the relative state ofthe level of a same-phase input terminal and the level of an anti-phase input terminal after becoming a first level signal through over-current protection by high-current trigger, so that the level state of an output terminal of the first comparator is maintained, and a processor can detect an overvoltage or over-current protection signal in time so as to implement protection to protect a motor.The over-current protection self-locking circuit avoids the situation that repeated loss of a power module is prone to occurring in a traditional over-current protection circuit, and the motor can beeffectively protected, and the service life of the motor and the service life of the air conditioner are prolonged.