The invention discloses an optical fiber embedded micro-fluidic chip based on SERS (Surface Enhanced Raman Scattering) detection and a detection device. The micro-fluidic chip comprises quartz glass,a micro-fluidic channel and an optical fiber SERS probe, wherein the micro-fluidic channel is prepared in the quartz glass; the optical fiber SERS probe is embedded into the quartz glass and comprisesa multimode fiber, a double pore capillary and an SERS substrate; one end of the double pore capillary is communicated with the micro-fluidic channel, and the middle part of two pores at the other end of the double pore capillary is removed to enable the two pores are communicated so that the sample solution can flow from one pore at one end of the double pore capillary and flow out of the otherpore; the multimode fiber and the other end of the double pore capillary are fused together; and the SERS substrate is prepared on the inner wall of the double pore capillary in situ. The detection device comprises an injection pump, an optical fiber embedded micro-fluidic chip, a laser light source, a spectrograph and a beam-splitting device. According to the optical fiber embedded micro-fluidicchip disclosed by the invention, the SERS detection stability and reliability can be effectively improved, and the requirements on the sample usage and the manual operation complexity can be reduced.