The invention belongs to the field of antibody medicine and relates to human-derived VEGFR2 resisting single-chain antibody and application thereof. The single-chain antibody is prepared from a heavy-chain variable area with a sequence shown in SEQ ID No.1 and a light-chain variable area with a sequence shown in SEEQ ID No.2; furthermore, the heavy-chain variable area and the light-chain variablearea are connected through flexible peptide, and an amino acid sequence of the flexible peptide is shown in SEQ ID No.3. The invention further discloses the application of the antibody in preparing tumor growth suppressing products. The antibody disclosed by the invention can serve as a medicine for clinical use of indications caused by neovascularization.