The invention discloses a large-area cooling plate temperature adjustment method for a large cryogenic compartment. The adjustment method includes pre-adjusting the wall of the cryogenic compartment,acquiring the flow rates of cooling plate groups of the wall, and adjusting the opening degree of a hand valve of each cooling plate group to make the flow rate of the wall uniform; acquiring the flowrates of cooling plate branches, and adjusting the opening degrees of branch hand valves to make the flow rates of the cooling plate branches uniform; injecting low-temperature gases into the wall ofthe adjusted cryogenic compartment. The adjustment method has the advantages that the cooling plate groups and the cooling plate branches of the wall are adjusted first to ensure uniformity of the flow rates of the cold plate groups and the cold plate branches, the low-temperature gases are injected into the wall of the adjusted cryogenic compartment and can be evenly distributed in the wall of the cryogenic compartment, and accordingly, the temperature uniformity of the space inside the large cryogenic compartment is improved.