The invention relates to a biaxially oriented polypropylene pre-coating substrate and a preparation method and application thereof. The biaxially oriented polypropylene pre-coating substrate comprisesa polypropylene surface layer, a polypropylene resin core layer and a functional layer and is prepared from the polypropylene resin surface layer, the polypropylene core layer and the functional layer through a co-extrusion biaxial stretching process, wherein resin of the functional layer contains an ethylene-vinyl acetate copolymer and an ethylene-octylene copolymer. The biaxially oriented polypropylene pre-coating substrate has the advantages that the functional layer is formed on a polypropylene film base material through co-extrusion, an EVA hot melt adhesive or a water-based adhesive canbe directly applied without applying a primer coating agent, a preparation process for a pre-coating film can be simplified, the coating amount of the adhesive can be decreased, and the production efficiency of the pre-coating film can be improved.