The invention provides an animal exposure experiment device. The device comprises a pollution box, a cleaning box, air pumps, sewage discharge valves, a collector, a cutting head, an efficient filter,air inlet pipes and air pumping pipes; the pollution box and the cleaning box are both connected to the outside through the air inlet pipes and the air pumping pipes, the pollution box and the cleaning box are connected to the air pumps through the air pumping pipes respectively, the sewage discharge valves are arranged at the bottoms of the pollution box and cleaning box respectively, the corresponding air inlet pipe of the pollution box is provided with the cutting head, the collector is disposed between the pollution tank and the corresponding air pump, and the corresponding air inlet pipeof the cleaning box is provided with the efficient filter. The provided animal exposure experimental device is simple in overall structure and convenient to operate, the concentration level of gaseous pollutants in the pollution box and cleaning box is identical to that of the outside, the device is more in line with the current actual situations of air pollution, and a certain reference value for measurement of the current air quality in China is achieved.