The invention discloses a corn ochrarin absorbing material and preparation and application methods thereof. The preparation method of the corn ochrarin absorbing material comprises mixing and firing tetrabutyl ammonium chloride, sodium sulfate and polyacrylamide-tetraethylenepentamine grafted chelating gel, during which the firing temperature is 120-150 DEG C, and the weight ratio of tetrabutyl ammonium chloride, sodium sulfate and polyacrylamide-tetraethylenepentamine grafted chelating gel is 1:7:2. The preparation method of the corn ochrarin absorbing material is rapid and convenient and applies special ion solution, salt and the grafted chelating gel, so that the prepared corn ochrarin absorbing material can achieve high targeting property and capturing efficiency. The corn ochrarin absorbing material achieves a corn ochrarin permeating rate up to 99.2% and is wide in linear range and low in detection limit and relative standard deviation, and can meets the national requirements oncorn ochrarin.