The invention relates to a preparation method of organic silicon micro-emulsion. The organic silicon micro-emulsion is prepared from an organic silicon polymer, a polyether modified polysiloxane mixture, a surfactant, an auxiliary emulsifier, a thickening agent and water. A turbine stirrer, an external circulating pump and an emulsifying machine commonly act on emulsion. In an emulsion preparationprocess, a hydrophilic emulsifier is mixed with the water at first; then a lipophilic emulsifier is added; finally, the organic silicon polymer is slowly added and the temperature-resisting and shearing-resisting organic silicon micro-emulsion is prepared through a method of controlling a grain diameter of each step. The emulsion provided by the invention can keep an original state in a working solution for a long period and has lasting performance.