The invention discloses a five-axis linkage machine tool dynamic and static error comprehensive detection method. The five-axis linkage machine tool dynamic and static error comprehensive detection method is characterized in that when accuracy detection is carried out on an accuracy detecting piece machined through a five-axis linkage machine tool, all feeding shaft actual movement positions of the machine tool in the machining process are monitored and analyzed, and dynamic errors are obtained; detection analysis is carried out on the actual molding outline face of the accuracy detecting piece, and actual errors are obtained; and the actual errors are coupling of the dynamic errors and static errors, the actual errors are decoupled, and static error distribution within the machining spacescope of the detecting piece is obtained. By means of the five-axis linkage machine tool dynamic and static error comprehensive detection method, through machining and detecting of the accuracy detecting piece, dynamic error and static error distribution along the processing track can be simultaneously obtained, five-axis linkage machine tool dynamic and static error comprehensive detection is achieved, and reference and basis are provided for error comprehensive compensation.