The invention provides a multi-antigen biomimetic influenza vaccine and a preparing method and application thereof. The multi-antigen biomimetic influenza vaccine comprises virus-like particle carrierprotein, surface antigens and luminal antigens; the surface antigens are distributed on the surface of the virus-like particle carrier protein, and the luminal antigens are distributed in internal cavities of virus-like particles. The multi-antigen biomimetic influenza vaccine structure simulates the natural spatial conformation of an influenza virus, multiple influenza-virus antigens are shown,the immunogenicity of the multiple influenza-virus antigens can be simultaneously played, a more-comprehensive immune effect is stimulated, and the immune protection effect of more-broad-spectrum anti-influenza viruses is provided.