The invention discloses a method for breeding a high-nodule nitrogen fixing transgenic plant. The nucleotide sequence as shown in SEQID NO:1 in a sequence table is subjected to overexpression in a plant body, and a transgenic plant having high nodule nitrogen fixing capacity compared with a normal plant is obtained. Experiment verifies that the transgenic plant is obtained through transforming anacceptor namely a glycine max plant through an overexpression vector constructed by the method, the nodule nitrogen fixing capacity of the glycine max can be notably improved, and the method has important actual significance in increasing the yield of the glycine max. The technical scheme of the method has actual value in the two respects of basic scientific research and farming and forestry application.