The invention discloses a refining method of octreotide. The refining method comprises the following step of using high-efficiency reversed liquid phase chromatography for carrying out reversed-phaseenrichment, reversed-phase salt conversion and reversed-phase purification on a crude octreotide solution to obtain a finished product, wherein the crude octreotide solution is obtained by oxidation of a reduced crude octreotide solution generated by solid-phase synthesis. A filler of high-efficiency reversed liquid phase chromatography is a super water-resistant filler. The method utilizes a one-step approach for online reversed-phase enrichment, reversed-phase salt conversion and reversed-phase purification to obtain a pure polypeptide product, the impurity removal rate of the crude octreotide product is high, the purity of an obtained pure octreotide product is high, and the production process is optimized, wherein an aqueous solution is adopted as the flow phase at the stages of columnbalance, sampling enrichment and salt conversion. The refining method is environmentally friendly and causes no pollution, and waste liquid flowing out can be directly subjected to sewage disposal and recycled.