The invention discloses a method for refining oxytocin [+Gly] impurities. The method comprises the step of sequentially performing reversed-phase enrichment, reversed-phase salt-turning and reversed-phase purification on an oxytocin [+Gly] impurity crude solution by adopting high-performance liquid reversed-phase chromatography, wherein super-water-resistant filler is used as filler in the high-performance liquid reversed-phase chromatography; and the reversed-phase enrichment, reversed-phase salt-turning and reversed-phase purification are completed in a one-step reversed-phase elution process. The method for refining oxytocin [+Gly] impurities is economical and environment-friendly since waste liquid generated in the purifying process is mostly wastewater and can be recycled through simple treatment in a sewage treatment plant.