The invention relates to a production method of a biodegradable nylon-wool blended yarn. The production method of the biodegradable nylon-wool blended yarn comprises the following steps of selecting materials of merino wool and biodegradable nylon fiber; soaking the materials, specifically, adjusting the temperature and humidity of a spinning workshop, soaking the merino wool with a diluted softening and smoothing agent of basok, spraying anti-static agent solution onto the nylon fiber, separately packing the merino wool and the nylon fiber, and stacking the packed materials in a sealed mode for 24-48 hours; drying the materials, specifically, performing high-temperature drying, washing and drying treatment sequentially on the soaked merino wool and the nylon fiber; doubling the merino wool and the nylon fiber at a winding speed of 310 m/min; twisting the materials, controlling the speed of a spinning roller and a spindle, the diameter of a steel ring and the forward and reverse degreeof twist of yarns to be twisted; lastly, performing roving, spinning, spooling, water washing and soaking, ultrasonic vibrating and washing, dewatering and drying. The production method of the biodegradable nylon-wool blended yarn can not only achieve the biodegradable function of the yarns, but also keep the excellent traits of the wool fiber as well as the high-strength and wear-resisting mechanical properties of the nylon fiber.