The invention provides a monorail vehicle rail installation joint. The monorail vehicle rail installation joint includes a foundation embedded part, an embedded anchor bolt and installation plates; the foundation embedded part is fixedly connected with the installation plates stacked on the foundation embedded part; the foundation embedded part is fixed in secondary grouting concrete by secondarygrouting, and the secondary grouting concrete is connected to the upper part of a concrete stand column cover beam; the embedded anchor bolt is embedded in the concrete stand column cover beam successively through the foundation embedded part and the secondary grouting concrete; and a rail beam is connected to the upper surface of the installation plate. The invention further provides a construction method of the monorail vehicle rail installation joint. The construction method includes installation and fixation of the foundation embedded part and the anchor bolt, installation of the rail beamand the secondary grouting. According to the monorail vehicle rail installation joint and the construction method thereof, the rail beam and the concrete stand column cover beam are connected by thewelding between the foundation embedded part and the installation plates, and engineering construction has greater fault tolerance and is convenient to adjust and maintain for a later period. At the same time, the concrete structure cast-in-place is adopted, and the problems that the transportation of the steel structure is difficult and lifting is not convenient are solved.