The invention relates to the technical field of cryogenic valves, in particular to an axial flow check valve under cryogenic working conditions. According to the cryogenic axial flow check valve, thetail of a flow guide component of the check valve is provided with an external thread segment which is in screw thread fit with the wall of a valve cavity; an external thread fixing component is arranged at the right end of the flow guide component and abuts against the flow guide component; at least one of the external thread segment of the flow guide component and the external thread fixing component is provided with an eccentric structure which is arranged in a radial eccentric mode, through the eccentric nut design of the external thread segment and the external thread fixing component, self-locking of the flow guide component is guaranteed, and the fabricated flow guide component is made to fail to be loosened in the cryogenic status using process; and in addition, the flow guide component and a valve body are manufactured separately, the casting process is good, the casting forming precision is high, and machining and manufacturing are convenient.