The invention provides a pressure-resistant optical detection flow cell, which can be used for analysis systems such as high performance liquid chromatography (HPLC), ultra-high performance liquid chromatography (UPLC) and flow injection analysis (FIA). The flow cell is only constituted by a flow cell support, a flanged polymer tube (PTFE or FEP or PFA), a quartz tube and a standard pressing cap screw structure; the flanged polymer tube is manufactured through a professional flanging device to ensure that as for the flanged tube, the end face is smooth, inner holes are smooth, and flanging approximates to be right-angled; and the end face of the quartz tube is polished, and the quartz tube and the flanged polymer tube are directly connected in a pressed mode to be sealed. According to theoptical detection flow cell, it is ensured that flow liquid only makes contact with the polymer tube and the quartz tube, inertia is ensured, and a sample is free of adsorption; zero dead volume exists in the sealed position of the optical detection flow cell, and peak broadening and peak tailing are lowered; and differing from an adhesive structure of a commercial flow cell, a pressing connectionsealing structure of the optical detection flow cell enables the flow cell to be resistant to the pressure of 3.5 Mpa.