The invention discloses Nocardiopsis sp. Y469 and a fermentation culture method thereof, and belongs to the technical field of biology. The Nocardiopsis sp. Y469 is separated from sediment of seabed at 3,700 meters in the Arctic Ocean. A strain is preserved at the China Center for Type Culture Collection of Wuhan University, Wuhan City, China on August 13, 2018, and the preservation number is CCTCC NO: M 2018536. The invention further provides the fermentation culture method of the Nocardiopsis sp. Y469; by comparing fermentation conditions such as different fermentation media, the fermentation conditions which can significantly improve the diversity of secondary metabolites are optimized, and the full exploration of the potential of the strain to produce new secondary metabolites is facilitated.