The invention belongs to the technical field of animal medicine preparations, and specifically relates to a compound propolis composition for treating dysentery of livestock and poultry. A preparationmethod and applications thereof are further disclosed. The composition is prepared by taking propolis, nutmeg, cassia bark, sangusis draconis, silktree albizia bark, diverse wormwood herb, pimpinellathellungiana, liquorice root, medicinal evodia fruit, tree peony bark, finger citron and millet sprout as raw materials. Based on the compatibility and combination of the above traditional Chinese medicines according to Chinese veterinary theories, and for the pathogenic factors and clinical symptoms of the dysentery of livestock and poultry, the composition can play a role of simultaneous treatment of principal and subordinate symptoms.