The invention discloses an antiseptic, anti-oxidation multi-effect active nano fresh-keeping film for cold fresh meat and a preparation method thereof. The raw materials of the fresh-keeping film comprise, in parts by weight, 1.00-20.00 parts of chitosan, 1.00-10.00 parts of sulfobutyl-beta-cyclodextrin, 0.50-10 parts of epsilon-polylysine, 1.00-25.00 parts of compound catechin, 100-300 parts of edible gelatin, 10-80 parts of glycerin, and 1-10 parts of transglutaminase. The provided compound catechin-epsilon-polylysine active nano fresh-keeping film for cold fresh meat is natural, safe and non-toxic, and environment-friendly, antioxidant properties of catechin and antibacterial properties of epsilon-polylysine are combined, good antioxidant and antibacterial properties are obtained, and sustained release of active substances is realized to maintain the properties of the film for a relatively long period of time. The fresh-keeping film is used for fresh-keeping of cold fresh meat, the problem that a fresh-keeping film containing a catechin substance brings bad color performance to a meat sample is solved, and further the shelf life of cold fresh meat is prolonged.