The invention provides an SVA (Seneca valley virus A)-PCV2 (porcine circovirus 2) fusion protein, a preparation method thereof, a gene, a biomaterial, an application and a vaccine, and relates to thetechnical field of biology. The SVA-PCV2 fusion protein comprises an SVA zone and a PCV2 zone, wherein the SVA zone comprises a VP1 polypeptide of an SVA, and the PCV2 zone comprises a Cap protein ofa PCV2. The SVA-PCV2 fusion protein has immunogenicity of the SVA and the PCV2, and a body can simultaneously generate immune response to the SVA and the PCV2. Based on the inventive concept, the invention further provides the gene for mainly encoding the SVA-PCV2 fusion protein, the biomaterial comprising the SVA-PCV2 fusion protein and the gene and the vaccine, wherein the vaccine has double immunogenicity of the SVA and the PCV2, and can achieve the purpose of preventing two diseases by one injection.