The invention provides a method for isolating and culturing suspension cells of dendrobium officinale. The method comprises the following steps of isolation and culture of root tissue cells of the dendrobium officinale, suspension culture of high-end granzyme active cells in a quiescent center and large-scale suspension culture. The method solves the problems that an isolated material of the dendrobium officinale is easy to brown, and scaled continuous culture cannot be achieved; the high-end granzyme active cells in the quiescent center of the root are screened, the proliferation is large under certain conditions, the cell activity is strong, and a stable large-scale dendrobium officinale cell suspension culture continuous amplification system is kept all the year round.